Ex vivo models of human ocular surface regeneration indicate that conjunctiva possess the capacity of restoring corneal epithelium
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چکیده
منابع مشابه
Characterization of Ex Vivo Expanded Oral Mucosal Epithelium Cells on Acellular Porcine Corneal Stroma for Ocular Surface Reconstruction
PURPOSE To ex vivo expand oral mucosal epithelium cells (OMECs) on acellular porcine corneal stroma (APCS) without using feeder cells and serum and to compare the morphologic and phenotypic characteristics of cultured oral cells on APCS to those of cells on deluded human amniotic membrane (HAM). METHODS SD rat oral mucosal biopsies were cultured on APCS and HAM. Reverse-transcription polymera...
متن کاملIn vivo models of human airway epithelium repair and regeneration
Despite an efficient defence system, the airway surface epithelium, in permanent contact with the external milieu, is frequently injured by inhaled pollutants, microorganisms and viruses. The response of the airway surface epithelium to an acute injury includes a succession of cellular events varying from the loss of the surface epithelium integrity to partial shedding of the epithelium or even...
متن کاملIn vivo effects of 5-FU on ocular surface epithelium following corneal wounding.
We evaluated the dose relationship between the antiproliferative and toxic effects of 5-fluorouracil (5-FU) on the ocular surface epithelium following experimental corneal epithelial wounding in rabbits. Central corneal epithelial defects 8 mm in diameter were made using n-heptanol. 5-FU (0.05 mg, 0.5 mg, or 5.0 mg per day in divided doses) or saline was applied topically for up to 18 days begi...
متن کاملKeratin 13 is a more specific marker of conjunctival epithelium than keratin 19
Introduction To evaluate the expression patterns of cytokeratin (K) 12, 13, and 19 in normal epithelium of the human ocular surface to determine whether K13 could be used as a marker for conjunctival epithelium. Methods: Total RNA was isolated from the human conjunctiva and central cornea. Those transcripts that had threefolds or higher expression levels in the conjunctiva than the cornea wer...
متن کاملHuman uroplakin Ib in ocular surface epithelium.
PURPOSE To investigate the expression and localization of the human gene encoding uroplakin Ib in ocular surface epithelium. METHODS The full-length cDNA of human uroplakin Ib was isolated from a cDNA library of human corneal epithelium, and the expression of uroplakin Ib in various tissues was examined by reverse transcription-polymerase chain reaction (RT-PCR). In cornea and conjunctiva, th...
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ژورنال
عنوان ژورنال: Acta Ophthalmologica
سال: 2021
ISSN: 1755-375X,1755-3768
DOI: 10.1111/j.1755-3768.2020.0099